By vantal The occurrence of depression is closely related to chronic stress. Stress can activate hypothalamus-adenohypophysis-adrenal axis, and make the body adapt to this state of stress, and the mice are fixed. However, under long-term stress, the sustained excitement of hypothalamus-adenohypophysis-adrenal axis will have a great impact on physical and mental health, and then lead to depression, which is considered to be an important mechanism of depression induced by chronic stress. At present, the commonly used animal models of depression, such as chronic unpredictable stress model, have a large workload and long modeling time.in fact suture kit It is more and more welcomed by our customers, and its market performance is gradually improving. https://pinnaclemedics.com/
Rat tail suspension method was originally used to prepare animal models of osteoporosis, but with the deepening of research, it has been confirmed that long-term rat tail suspension can be used as a low-level and sustained chronic stress, which can further affect the functions of the central nervous system and immune system. In this experiment, we used this method to prepare the model of mice with depression. The results showed that both model 1 and model 2 showed depression-like behavior, and compared with the control group, the weight of model 1 and model 2 decreased significantly after modeling.
Experimental white mouse fixing frame
Therefore, from the behavioral point of view, it is safe, reliable, feasible and effective to prepare mouse depression model by tail suspension. Thyroid hormone not only participates in the growth and metabolism of the body, but also plays an important regulatory role as an important substance to maintain the normal excitability of the central nervous system. It is closely related to the functions of memory, spirit and emotion in experimental mice.
Studies have shown that hypothyroidism is a high-risk factor for depression, and most patients with depression are accompanied by hypothyroidism in clinic. However, some studies have found that some patients with depression have slight hyperthyroidism l5s, so different studies have different views on the correlation between depression and thyroid function. Experimental white mouse fixing frame.
In this experiment, it was found that the serum FT4 content in model 1 and model 2 groups was significantly lower than that in the control group, and the decline in model 2 group was more obvious. In addition, the pathological staining in this experiment showed that the pathological morphology of thyroid in model group 2 showed abnormal changes, mainly in the flat and different sizes of thyroid follicular epithelium, suggesting hypothyroidism. In this experiment, there is no significant difference in serum TSH and FT3 contents among the three groups after modeling, which may be due to the improvement of deiodinase activity, so it can promote the transformation from T4 to T3 in a compensatory way. Experimental white mouse fixing frame.
MAO has the function of decomposing monoamine neurotransmitters, and MAO inhibitors have become classic antidepressants used in clinical treatment. Previous studies have reported that the decrease of monoamine neurotransmitters such as NE, 5- hydroxytryptamine and dopamine in hypothalamus caused by various reasons is closely related to the occurrence of depression. The level of 5- hydroxytryptamine in hypothalamus of chronic stress model rats decreased significantly, and the level of 5- hydroxytryptamine in hypothalamus of model rats was effectively increased after standardized antidepressant treatment. Experimental white mouse fixing frame.
Experimental mouse mouth gag
This experiment showed that the activity of MAO in hypothalamus of model group 1 and model group 2 was significantly higher than that of control group, and the contents of 5- hydroxytryptamine and NE were significantly lower than that of control group. The results suggest that the increase of MAO activity and the decrease of NE and 5- hydroxytryptamine levels in hypothalamus of rats after the establishment of animal model by tail suspension may be one of the main reasons for the depression-like behavior of model mice.
To sum up, the rat tail suspension method can be used to prepare the depression model of experimental mice simply and effectively: the body is subjected to long-term and chronic stimulation, which makes it in a state of chronic stress for a long time, and further leads to emotional disorders and a series of pathological and physiological changes in the neuroendocrine system. The animal behavior performance and changes in monoamine neurotransmitters in hypothalamus are similar to those of the classic depression animal model, suggesting that this method can successfully prepare the depression animal model.
Moreover, this method can reduce the function of HPT axis of experimental mice, but it can be compensated by promoting the transformation from T4 to T. However, the specific mechanism is still unclear and further analysis is needed in the future.