By vantal Establishment of acute limb crush ischemia-reperfusion injury model in rats with constant pressure and volume of mouth gagperiod suture kit Many adjustments have also been made, and the direction of product research and development has been reversed, in order to better meet the market demand. https://pinnaclemedics.com/
Ischemiareperfusioninjury (IRI) is a common pathological state, which is characterized by limited blood supply to organs or tissues, and then blood perfusion and oxygen supply are resumed. However, after blood perfusion and oxygen supply are restored, serious inflammatory reaction and tissue damage are aggravated.
Rat fixator
Sewell et al. first discovered this phenomenon in canine coronary artery ligation experiment, and then Jennings et al. observed and formally put forward the concept of ischemia-reperfusion injury through canine coronary artery occlusion experiment. This kind of injury is very common and has a wide range of effects. For example, cardiac arrest often leads to ischemia of multiple organs, and reperfusion injury can be caused after the blood flow of organs is restored after resuscitation; After IRI, a single organ can also lead to the inflammatory activation of other organs, and the rat mouth opener will eventually lead to multiple organ dysfunction.
The occurrence of IRI involves many complicated pathophysiological processes: Abnormal Na+/Ca2+ exchange and biofilm damage increase intracellular Ca2+,inhibit mitochondrial function, promote the generation of oxygen free radicals, and destroy nucleic acids and chromosomes; Activation of cell death program leads to apoptosis, autophagy cell death and necrosis; Induce autoimmune reaction, activate natural antibody recognition system and complement system, and cause tissue damage; ∠ Increase the single electron reduction of oxygen in mitochondria, the oxidation ability of catecholamine, activate neutrophils, etc., and the mouthparts of rats cause tissue cell damage and death.
Limbischemia reperfusion injury (LIRI) is very common in the field of trauma surgery. Skeletal muscle ischemia for a short time can cause irreversible damage, which not only affects the survival and function of ischemic limbs, but also causes systemic inflammatory response syndrome (SIRS), which leads to multipleorganfailure (MOF) in rats with multiple organ failure, even life-threatening.
In recent years, with the further in-depth discussion and extensive research on IRI theory, animal experiments and clinical trials, LIRI has attracted widespread attention from scholars at home and abroad. Therefore, it is of great practical significance to construct a standardized LIRI model for studying its pathogenesis and prevention and treatment points. At present, there is no unified standard method for establishing LIRI animal model. The commonly used LIRI models mainly include the femoral artery model with non-invasive vascular clamping, the lower limb vascular external banding model, the rat mouth opener, and the small animal inflatable tourniquet blocking model.
Rat mouth gag
However, the above methods have different defects: the vascular clamping model is traumatic, complicated to operate, takes a long time, and is prone to incision infection and neurovascular injury; The model of external ligation of lower limb blood vessels is not uniform and easy to loosen. The tourniquet model is difficult to fix, the tourniquet is easy to leak and lose pressure, and the stability of the model is poor.
In this study, based on the previous models, an acute SpragueDawley(SD) rat model of mouth organ crush ischemia-reperfusion injury was established by improving the methods of stabilizing the extrusion pressure and the constant compressed muscle volume. The validity and stability of the model were evaluated by routine physiological and biochemical indicators and histological observation, with the aims of: building a stable and highly repeatable LIRI animal model; To explore the effect of LIRI on distant organs and the specific mechanism of related injuries; Evaluate the effective methods to prevent LIRI, and explore the specific mechanism and intervention conditions (intervention degree, time node, etc.) of effective treatment measures (such as hemofiltration) to improve organ function and prognosis.